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Lab Protocols

Analysis of surface lipid-phenolic polyesters (cutin, suberin) by base-catalyzed depolymerization

Analysis of surface lipid-phenolic polyesters (cutin, suberin) by acid-catalyzed depolymerization

GC-MS-based analysis of chloroform extracted suberin-associated root waxes from Arabidopsis and other plant species

Seed coat permeability test: tetrazolium penetration assay

Protein extraction from leaves, SDS-PAGE, coomassie staining, and Western blot
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